Figure 7
Expression analysis of phenylpropanoid and flavonoid pathway genes in flowers of T 2 transgenic tobacco lines carrying MdMYB3 using qRT-PCR. Normalization was adjusted to expression of an apple actin gene, and values are means of three technical replicates. Transcript levels in flowers of three transgenic lines were quantified relative to those present in flowers of wild-type plants. Abbreviations correspond to the following: CHI: chalcone isomerase; CHS: chalcone synthase; F3H: flavonoid 3-hydroxylase; F3′H: flavonoid 3′-hydroxylase; FLS: flavonol synthase; DFR: dihydroflavonol reductase; LAR: leucoanthocyanidin reductase; ANS: anthocyanidin synthase; UFGT: glucose transferase; ANR: anthocyanidin reductase; C4H: cinnamate-4-hydroxylase; 4CL: 4-coumaroyl-CoA ligase; PAL: phenylalanine ammonia lyase; COMT: caffeoyl-CoA o-methyltransferase; and CAD: cinnamyl alcohol dehydrogenase.