Figure 5

Effect of heat stress on P. patens wild type and double knockout mutant. (A) Phenotypic comparison of WT and dKO-1 plants grown in optimal conditions (Ctrl) and incubated at 37°C for 7 days. Plant colonies were subsequently transferred to normal medium, and the stress recovery process was monitored during time and photographed at 6, 14 and 17 days. (B) Total chlorophyll content (mg/g/fresh weight) per colony was determined from non-stressed controls (Ctrl), heat stressed plants for 7 days (37°C) and at the indicated time points of recovery in standard growth medium. (C) Dry weight per colony (mg) of control (Ctl), stressed (37°C) and plants allowed to recover in optimal growth conditions for 17 (rec). The values shown are means from one representative technical replicate. Error bars indicate SD (n = 15). Three biological replicates were carried out. Significant differences of at least 0.05 confidence level between the wild-type and the KO lines are marked by *.