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Table 1 Cytological, morphological and fruit quality parameters of diploid and allotriploid donors and the induced-autopolyploid lines

From: Morphological, cytological and metabolic consequences of autopolyploidization in Hylocereus (Cactaceae) species

  Hylocereus Autotetraploid Allotriploid Autohexaploid
Parameter monacanthus D-27 S-75 D-2.3
Cytological parameters     
Estimated ploidy level 2n 4n 3n 6n
Genome size (pg ±SE) 4.2 ±0.1 8.0 ±0.4* 5.9 ±0.3 13.7 ±0.1*
Stomata diameter (µm ±SE) 53.1 ±0.2 56.0 ±0.2* 63.7 ±0.3 69.0 ±0.3*
Stomata density (±SE) 13.6 ±0.3 12.6 ±0.3* 9.4 ±0.2 8.1 ±0.2*
Number of ovules (±SE) 5036 ±159 3408 ±185* 3530 ±107 3472 ±187
Pollen grain size (µm ±SE) 84.9 ±0.2 107.9 ±0.4* 97.6 ±0.6 122.7 ±0.5*
Pollen stainability (% ±SE) 97.1 ±0.2 86.6 ±1.4* 11.1 ±0.7 52.2 ±4.8*
Tetrads (% ±SE) 99.8 ±0.2 78.3 ±2.4* 74.6 ±0.4 30.3 ±3.9*
Polyads (% ±SE) 0.0 ±0.0 18.9 ±2.2* 9.0 ±2.3 61.1 ±2.7*
Triads (% ±SE) 0.0 ±0.0 2.8 ±0.7* 16.0 ±2.1 6.5 ±1.1*
Dyads (% ±SE) 0.2 ±0.2 0.0 ±0.2 0.3 ±0.2 0.7 ±0.2
Monads (% ±SE) 0.0 ±0.0 0.0 ±0.2 0.1 ±0.1 1.4 ±0.5*
Morphological parameters     
Fruit weight (gr ±SE) 333 ±17 267 ±18* 195 ±11 89 ±14*
Peel weight (gr ±SE) 158 ±9 138 ±9 77 ±4 47 ±5*
Flesh weight (gr ±SE) 175 ±10 129 ±10* 118 ±8 42 ±9*
Flesh/Peel ratio (±SE) 1.14 ±0.05 0.95 ±0.08 1.53 ±0.06 0.85 ±0.11*
Black-coat seed number (±SE) 3263 ±201 2190 ±258* 1681 ±113 295 ±37*
Brown-coat seed number (±SE) 0 0 569 ±79 371 ±71
Black-coat seed weight (mg ±SE) 2.57 ±0.02 2.92 ±0.04* 3.86 ±0.06 2.29 ±0.05*
Seed germination (% ±SE) 89 ±1.4 81 ±2.3* 50 ±2.7 30 ±2.5*
Fruit quality parameters     
pH (±SE) 3.59 ±0.05 3.33 ±0.06 3.36 ±0.06 2.72 ±0.04*
TSS (% ±SE) 14.1 ±0.31 13.8 ±0.10 16.2 ±0.30 13.6 ±0.30*
Water content (% ±SE) 83.51 ±0.6 84.12 ±0.3 83.46 ±0.1 84.70 ±0.1
  1. *represent statistical significance differences between each of the induced-autopolyploid line to its respective donor line. Statistical analysis was calculated according to the Student’s t-test analysis, P ≤ 0.05. TSS, total soluble solids.