Figure 7

Kinetic RT-PCR validation of microarray analyses for a representative subset of genes. cDNA was synthesized from the areal portion of well watered plants (Stage 0) and at visible drought (Stage 2). Amplification of the housekeeping gene encoding polypyrimidine tract-binding protein1 (PTBpa, AT3G01150) was used as control to normalize PCR expression data. All values represent the averages of three biological replicates, each analyzed three times (technical replicates). Values from microarray expression are ratios of averaged, normalized fluorescent signals.