Figure 5From: Mechanical stress in Arabidopsis leaves orients microtubules in a 'continuous’ supracellular patternApplication of pressure at a specific location on the leaf. External pressure is exerted by a up-side-down placed glass petri dish (50 mm) (a). An 19 DAS old leaf can be divided in three distinct zones as shown on image b: zone of direct application of the pressure (grey), zone of microtubule response (blue) and the non-responding parts of the leaf (green). Representative images are shown in c for all three locations. (scale bar = 50 μm).Back to article page