Figure 2

Calcium channel blocker and calcium chelators abolish WIR. Droplets containing different concentrations of verapamil (10 μM, 100 μM, 1 mM, 10 mM), EGTA (1 mM, 10 mM, 50 mM, 100 mM) and oxalate (100 μM, 1 mM, 10 mM, 50 mM) were applied on leaves for 3 hours. After droplet removal, leaves were wounded to induce WIR and inoculated with B. cinerea spores on the same site where inhibitors were applied. After three days of incubation in covered trays, lesion diameters were measured. Black: non-wounded leaves. Grey: leaves wounded right after inhibitors removal to induce WIR. Asterisks represent significant differences using Student's t test relative to water-treated control; *P < 0.05, **P < 0.01.