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Table 5 PSSM/Sift scores for CAD mutant lines showing category 1 brown midrib phenotype

From: PT-Flax (phenotyping and TILLinG of flax): development of a flax (Linum usitatissimumL.) mutant population and TILLinG platform for forward and reverse genetics

[EMS]

Family

No. Indiv 1/total*

Mutation (Nt)

Mutation (AA)

PSSM difference

Sift score

0,3

645

3/3

G1734A

E351K

11.0

0.09

 

759

1/3

G1218A

V252I

7.3

0.11

 

823

1/5

G1026A

G188R

30.4

0.00

 

1021

1/4

G990A

G176R

_

0.00

 

1411

1/3

G936A

A158T

11.0

0.09

 

1564

1/1

C1303T

P280L

_

0.00

0.75

814

1/1

C999T

Q179Stop

damaging

damaging

 

877

1/3

C1079T

H205Q

8.0

0.01

 

884

1/2

G1137A

G225R

12.8

0.09

  1. PSSM scores and Sift scores were obtained with PARSESNP and SIFT programs, respectively. Scores in bold text are predicted to be damaging for protein activity while scores in normal text are predicted to be without effect. Mutations with no scores result from the lack of alignment block for the given position. *Number individual plants showing category 1 phenotype/total number plants phenotyped per family.