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Table 2 Effects of ORCA2 overexpression on the levels of selected metabolites in the TIA and feeder pathways

From: The ORCA2 transcription factor plays a key role in regulation of the terpenoid indole alkaloid pathway

Transgene

Result

Tryptophan

BD

Tryptamine

+

Loganin

=

Secologanin

-

Strictosidine

-

Ajmalicine

+

Serpentine

+

Catharanthine

+

Lochnericine

=

Hörhammericine

-

Tabersonine

-

16-hydroxytabersonine

+

19-hydroxytabersonine

+

Vindoline

BD

Vinblastine

BD

Vincristine

BD

  1. “+” = level of indicated metabolite was higher at one or more time points when ORCA2 was overexpressed; “-” = level of indicated metabolite was lower at one or more time points when ORCA2 was overexpressed; “=” indicates no significant change in metabolite level when ORCA2 was overexpressed; “BD” indicates the metabolite was below detection levels.