Figure 6

Time course analysis of transcript and metabolite levels from the terpenoid pathway. Results shown are the: uninduced ORCA2-OE line (□, solid line), 0.02% ethanol-induced ORCA2-OE line (■, solid line), uninduced control line (∆, dashed line) and 0.02% ethanol-induced control line (▲, dashed line). The relative mRNA levels are presented as ∆∆CT. Results depicted are as follows: A) DXS transcript levels, B) G10H transcript levels, C) CPR transcript levels, D) LAMT transcript levels, E) loganin levels and F) secologanin levels. Results for transcript levels are the average ∆∆CT values of three biological replicates, with two technical replicates for each biological replicate. Results for metabolite levels are the averages of three biological replicates. Error bars indicate standard deviations. Transcript levels of the indicated gene in the induced versus uninduced ORCA2-OE cultures differed at the same time point with: * = p ≤ 0.05, ** = p ≤ 0.01 according to a Student’s t-test. Similarly, metabolite levels differed with: * = p ≤ 0.1, ** = p ≤ 0.05 according to Student’s t-test. Student’s t-test results for the induced versus uninduced cultures of the control line are not depicted.