Figure 5

Time course analysis of ASα and TDC transcript and tryptamine metabolite levels. Tryptophan levels were below detection thresholds. Results shown are the: uninduced ORCA2-OE line (□, solid line), 0.02% ethanol-induced ORCA2-OE line (■, solid line), uninduced control line (∆, dashed line) and 0.02% ethanol-induced control line (▲, dashed line). A) Relative ASα transcript levels are presented as ∆∆CT. B) Relative TDC transcript levels are presented as ∆∆CT. Positive ∆∆CT values indicate that transcript levels for the indicated gene are higher in the indicated hairy root line grown for the indicated time under the indicated conditions than in the uninduced control line at 0 h. Conversely, negative ∆∆CT values indicate that transcript levels are lower for the indicated gene in the indicated hairy root line grown for the indicated time under the indicated conditions than in the uninduced control line at 0 h. Results for transcript levels are the average ∆∆CT values of three biological replicates, with two technical replicates for each biological replicate. C) Tryptamine levels are depicted. Results for metabolite levels are the averages of three biological replicates. Error bars indicate standard deviations. Transcript levels of the indicated gene in the induced versus uninduced ORCA2-OE cultures differed at the same time point with: * = p ≤ 0.05, ** = p ≤ 0.01 according to Student’s t-test. Similarly, metabolite levels differed with: * = p ≤ 0.1, ** = p ≤ 0.05 according to Student’s t-test. Student’s t-test results for the induced versus uninduced cultures of the control line are not depicted.