Figure 3From: The ORCA2 transcription factor plays a key role in regulation of the terpenoid indole alkaloid pathwayRelative ORCA2 transcript levels in five ORCA2 transgenic hairy root lines. ORCA2 transcript levels were assayed in five transgenic hairy root lines transformed with the binSRNA-ORCA2 and pPZPROL constructs (OR6, OR13, OR17, OR21 and ORCA2-OE) and in a control line carrying only the pPZPROL construct (Control). ORCA2 transcript levels were measured in these lines under induced and uninduced conditions, 10 h after induction with 0.5% ethanol. Relative mRNA levels are presented as ∆∆CT. ∆∆CT = ∆CTuninduced control line - ∆CTother line, where ∆CT = CT ORCA2 - CT EF1 and “other line” is the induced control line or uninduced or induced cultures of one of the binSRNA-ORCA2 containing lines. Positive ∆∆CT values indicate that ORCA2 transcript levels are higher in the indicated hairy root line grown under the indicated conditions than in the uninduced control line. Conversely, negative ∆∆CT values indicate that ORCA2 transcript levels are lower in the indicated hairy root line grown under the indicated conditions than in the uninduced control line. Results are the average ∆∆CT value of three biological replicates, with two technical replicates for each biological replicate. Error bars indicate standard deviations. ** indicates that ORCA2 transcript levels in the induced versus uninduced cultures of a hairy root line carrying the binSRNA-ORCA2 construct differed by p ≤ 0.01, according to Student’s t-test.Back to article page