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Figure 3 | BMC Plant Biology

Figure 3

From: The ORCA2 transcription factor plays a key role in regulation of the terpenoid indole alkaloid pathway

Figure 3

Relative ORCA2 transcript levels in five ORCA2 transgenic hairy root lines. ORCA2 transcript levels were assayed in five transgenic hairy root lines transformed with the binSRNA-ORCA2 and pPZPROL constructs (OR6, OR13, OR17, OR21 and ORCA2-OE) and in a control line carrying only the pPZPROL construct (Control). ORCA2 transcript levels were measured in these lines under induced and uninduced conditions, 10 h after induction with 0.5% ethanol. Relative mRNA levels are presented as ∆∆CT. ∆∆CT = ∆CTuninduced control line - ∆CTother line, where ∆CT = CT ORCA2 - CT EF1 and “other line” is the induced control line or uninduced or induced cultures of one of the binSRNA-ORCA2 containing lines. Positive ∆∆CT values indicate that ORCA2 transcript levels are higher in the indicated hairy root line grown under the indicated conditions than in the uninduced control line. Conversely, negative ∆∆CT values indicate that ORCA2 transcript levels are lower in the indicated hairy root line grown under the indicated conditions than in the uninduced control line. Results are the average ∆∆CT value of three biological replicates, with two technical replicates for each biological replicate. Error bars indicate standard deviations. ** indicates that ORCA2 transcript levels in the induced versus uninduced cultures of a hairy root line carrying the binSRNA-ORCA2 construct differed by p ≤ 0.01, according to Student’s t-test.

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