Figure 5From: The bZIP protein from Tamarix hispida, ThbZIP1, is ACGT elements binding factor that enhances abiotic stress signaling in transgenic ArabidopsisThe constitutive expression of ThbZIP1 decreases ROS levels and enhanced GST activity. A, B: The leaves from Col-0 and transgenic plants were pretreated with NaCl, mannitol, or ABA, and were stained with NBT to visualize O2-(A), or were stained with DAB to visualize H2O2(B). C: Analyses of ROS production in intact guard cells of transgenic and Col-0 plants indicated by H2DCF-DA. Epidermal peels were loaded with H2DCF-DA after the incubation with 10 μM ABA or 150 mM NaCl for 2 h. (1) peel cells imaged under bright field; (2) ROS in guard cells were detected by H2DCF-DA (ROS shown as green fluorescence); (3) Chloroplast in the leaves that shown as red fluorescence; (4) merge of bright field and fluorescence (chloroplast shown as red fluorescence). D: Detection of ROS accumulation in root tips of transgenic and Col-0 plants using H2DCF-DA staining method. E: Measurement of H2O2 level in transgenic and Col-0 plants. F: Comparison of GST activity between Col-0 and ThbZIP1-transformed plants. All experiments were repeated three times. Data are means ± SD from three independent experiments. * Significant (t test, P < 0.05) difference compared with Col-0 plants.Back to article page