Figure 5

Variation in transcript levels of tps10 and tps23 between epidermis and inner leaf tissues. Samples from the isolated epidermis and complete leaf of maize variety Delprim were compared after herbivore damage (dam) and in undamaged control plants (ctr). The epidermis was carefully stripped from the leaves and RNA was prepared from epidermis tissue as well as from the complete leaf containing the epidermis and inner tissues. The samples were blotted and probes specific for tps10 and tps23 were used for hybridization. The 28S RNA is shown as a loading control on an ethidium bromide stained agarose gel.