Table 1 Effect of pH, Ca 2+ /CaM and TFP on GAD activity in cell-free apple extracts
From: Calmodulin-dependent and calmodulin-independent glutamate decarboxylases in apple fruit
Treatment | pH | ||||
|---|---|---|---|---|---|
5.0 | 5.5 | 6.0 | 6.5 | 7.0 | |
nmol mg prot-1 min-1 | |||||
+Ca2+/CaM -TFP | 7.48±0.58a1 | 14.69±2.92a | 12.91±0.74a | 3.66±1.18a | 0.54±0.21a |
+Ca2+/CaM + TFP | 4.81±0.97b | 9.21±1.36a | 8.59±1.56b | 0.86±0.09b | 0.09±0.02b |
-Ca2+/CaM -TFP | 3.73±0.56b | 9.91±2.21a | 8.63±0.33b | 1.03±0.09b | 0.11±0.06b |
-Ca2+/CaM + TFP | 4.62±0.65b | 8.76±1.30a | 7.39±1.23b | 0.68±0.09b | 0.07±0.01b |
% Ca2+/CaM stimulation2 | 62 | 68 | 75 | 440 | 670 |