Figure 4

Impact of pH and Ca²⁺/calmodulin on the spectral properties of various recombinant apple and Arabidopsis GADs. These properties were determined on most of the purified proteins studied in Figure 3. The prefix H on some protein designations represents the 6-His motif. Absorbance at 338 nm and 415 nm, respectively, represents the enolimine and and ketoenamine forms of the internal aldimine. Each row of panels represents a different protein in the absence (A, C, E, G, I) and presence (B, D, F, H, J) of CaM.