Skip to main content

Table 3 Prolamins and non-gluten contents, gliadins and glutenins contents, total protein and starch contents, SDS sedimentation test and 1000 seed weight of transgenic and wild-type lines

From: Integration of promoters, inverted repeat sequences and proteomic data into a model for high silencing efficiency of coeliac disease related gliadins in bread wheat

     

Gliadin (μg/mg of flour)

Glutenin (μg/mg of flour)

Gli/Glu

(μg/mg of flour)

Protein (%)

Starch (%)

SDSS (mL · g − 1)

Weight 1000 (g)

Genotype

Line

Constructs

Promoter

iTarget

ω

α

γ

Total

LMW

HMW

Tota

Prolamins

Non-gluten

BW208

Wild type

Control

C

C

11.89

39.59

27.76

78.24

15.51

8.05

23.56

3.31

101.80

26.66

14.21

55.37

12.54

44.25

 

A1152

pGhpg8.1 + pDhpg8.1

GD

g

14.98

45.82

1.78***

61.59

18.09*

9.61

27.70

2.24

89.29

45.47

14.90

54.77

13.49

46.79

 

A1158

pGhpg8.1 + pDhpg8.1

GD

g

22.56

56.05

2.18***

79.78

17.61

10.01

27.62

2.83

107.40

25.28

14.67

54.83

11.88

46.86

 

A1406

pGhpg8.1 + pDhpg8.1

GD

g

18.19

57.84

1.67***

76.70

14.47

11.39***

25.86

2.94

102.56

53.06

17.17***

51.74

9.62***

39.84**

 

C655

pDhpg8.1

D

g

16.94

49.20

5.29***

70.44

19.59***

9.38

28.98

2.38

99.41

33.23

14.72

54.00

13.70

41.56

 

C657

pDhpg8.1

D

g

25.12

63.24

7.25***

94.61

17.92*

8.88

26.80

3.51

121.41

7.75

14.27

54.48

12.39

44.21

 

D445

pDhpg8.1

D

g

41.54*

78.41

2.17***

121.12

21.14***

11.42**

32.55***

3.73

153.68

-7.61

16.16***

52.68

13.55

34.8***

 

D577

pGhpg8.1

G

g

18.50

48.90

4.72***

71.13

18.48**

9.79

28.27

2.48

99.39

35.06

14.87

54.46

12.85

42.65

 

D623

pDhpg8.1

D

g

16.27

41.91

3.81***

60.99

18.78***

9.81

28.60

2.12*

89.58

45.63

14.92

54.66

12.39

44.50

 

D682

pGhpg8.1

G

g

24.02

56.85

4.14***

84.01

19.01***

9.72

28.72

2.95

112.74

15.59

14.20

54.77

13.02

45.36

 

28A

pDhp_ω/α

D

o

7.29***

17.13***

5.98***

29.39***

20.22***

16.70***

36.92***

0.82***

66.31**

58.41

13.81

55.21

12.56

39.45***

 

28B

pDhp_ω/α

D

o

6.39***

13.46**

5.09***

23.94***

19.95***

18.36***

38.31***

0.60***

62.26***

64.38

13.99

53.92

12.57

42.35

 

D770

pGhp_ω/α

G

o

6.25***

10.31***

2.95***

18.50***

17.91*

19.85***

37.76***

0.49***

56.27***

78.30*

14.82

55.03

12.18

39.56**

 

D783

pDhp_ω/α

D

o

5.05***

10.87***

4.21***

19.13***

19.6***

17.00***

36.6***

0.53***

55.73***

73.40*

14.26

55.98

12.36

42.56

 

D793

pGhp_ω/α

G

o

5.84***

5.78***

1.39***

12.01***

10.01***

14.34***

24.36

0.50***

36.37***

95.60***

14.48

55.68

8.55***

40.66*

 

D894

pGhp_ω/α

G

o

5.24***

9.86***

2.87***

16.98***

18.74***

16.37***

35.11***

0.50***

52.10***

78.72*

14.45

54.70

12.08

41.52

 

E33

pDhpg8.1 + pDhp_ω/α

D

go

5.88***

8.73***

1.66***

15.27***

15.57

19.86***

35.44***

0.43***

50.71***

90.83**

15.63

54.68

11.94

42.22

 

E35

pDhpg8.1 + pDhp_ω/α

D

go

6.47***

10.16***

1.75***

17.38***

16.45

18.45***

34.90***

0.50***

52.28***

80.44*

14.65

54.31

12.27

40.84*

 

E39

pDhpg8.1 + pDhp_ω/α

D

go

6.55***

14.88***

1.99***

22.41***

15.47

11.60***

27.07

0.84***

49.49***

93.16**

15.65

52.81

10.25***

37.94***

 

E42

pDhpg8.1 + pDhp_ω/α

D

go

5.51***

9.40***

1.29***

15.2***

11.66**

10.07

21.72

0.71***

36.92***

97.86***

14.90

51.78

8.98***

36.51***

 

E76

pDhpg8.1 + pDhp_ω/α

D

go

6.38***

3.70***

1.05***

10.12***

6.72***

13.04***

19.76

0.53***

29.88***

103.69***

14.77

53.44

7.18***

33.88***

 

E82

pDhpg8.1 + pDhp_ω/α

D

go

6.87***

4.14***

1.04***

11.06***

6.08***

9.29

15.37***

0.76***

26.43***

104.56***

14.46

53.28

6.23***

35.78***

 

E83

pDhpg8.1 + pDhp_ω/α

D

go

6.06***

3.15***

1.02***

9.24***

4.99***

8.47

13.46***

0.68***

22.70***

115.74***

15.28

50.91*

6.93***

32.61***

BW2003

Wild type

Control

C

C

12.87

28.15

22.93

62.95

13.93

10.83

24.76

2.53

87.70

39.21

14.01

57.48

11.84

44.65

 

22A

pGhpg8.1

G

g

28.25

49.06

3.74***

80.06

18.39***

16.87***

35.25***

2.21

115.31

18.58

14.87

55.85

14.62***

45.03

 

22C

pGhpg8.1

G

g

20.79

39.08

2.90***

61.76

17.61***

14.72*

32.33**

1.89

94.10

42.64

15.15

55.68

14.11***

46.71

 

24A

pGhpg8.1

G

g

36.51

59.00

5.02***

99.53

18.00**

15.06*

33.06*

3.10

132.59

55.73

16.08·

54.60

13.71**

40.62

 

24B

pGhpg8.1

G

g

18.90

34.64

2.75***

55.29

15.47

12.74

28.21

1.97

83.50

53.22

14.68

55.65

11.98

43.99

 

C217

pDhpg8.1

D

g

25.01

45.26

9.03***

78.29

15.92

13.64

29.55

2.50

107.85

24.26

14.70

56.56

12.91

43.33

 

D598

pDhpg8.1

D

g

16.82

33.07

6.91***

55.80

14.90

11.87

26.76

2.07

82.56

53.87

15.12

56.67

12.21

43.01

 

D715

pGhpg8.1

G

g

17.99

34.96

4.94***

56.90

16.99**

14.34·

31.33*

1.81*

88.23

53.77

15.68

56.71

13.75***

47.96

 

D716

pGhpg8.1

G

g

18.38

35.48

2.84***

55.70

16.67**

14.62*

31.28*

1.77

86.98

40.84

14.16

54.49

14.04***

48.63*

 

D815

pGhpg8.1

G

g

16.95

32.62

2.94***

51.51

15.84

12.61

28.44

1.79

79.95

56.99

14.85

57.19

13.08

38.01***

 

D874

pDhp_ω/α + pGhp_ω/α

GD

o

7.17***

5.22***

3.18***

14.58***

6.29***

19.96***

26.25

0.57***

40.83***

95.71*

15.06

54.5

8.95***

39.88**

 

D876

pDhp_ω/α + pGhp_ω/α

GD

o

6.17***

5.36***

3.12***

13.65***

5.96***

19.87***

25.83

0.54***

39.48***

95.27*

14.89

54.68

8.94***

39.29***

 

E140

pDhp_ω/α

D

o

6.43***

10.49***

6.29***

22.21***

14.42

19.93***

34.35***

0.65***

56.56***

76.2

14.7

56.45

12.24

41.53

 

E146

pDhp_ω/α

D

o

8.57***

13.20***

8.15***

28.92***

13.17

21.73***

34.90***

0.80***

63.81**

70.35

14.85

54.61

12.05

42.49

 

E122

pDhpg8.1 + pDhp_ω/α

D

go

7.42***

12.57***

3.32***

22.3***

12.62

18.99***

31.61

0.70***

53.91***

103.37*

17.44***

52.19**

11.79

34.32***

 

E93

pGhpg8.1 + pGhp_ω/α

G

go

7.03***

7.70***

2.15***

15.88***

8.52***

15.62**

24.15

0.67***

40.03***

98.71**

15.33

54.64

9.38***

38.65***

 

E96

pGhpg8.1 + pGhp_ω/α

G

go

7.13***

7.09***

2.03***

15.24***

7.56***

17.38***

24.94

0.62***

40.18***

94.37*

14.89

54.57

9.33***

41.62·

  1. Promoter, the promoter used to drive the silencing fragments; C, control line; G, γ-gliadin promoter; D, H-hordein promoter; GD, the γ-gliadin and D-hordein promoter; iTarget, silencing fragments; g, γ-gliadin silencing fragment; o, ω/α-gliadin silencing fragment; go, both silencing fragments; ω, ω-gliadins; α, α-gliadins; γ, γ-gliadins; total, total gliadin content; HMW, high molecular weight; LMW, low molecular weight; Gli/Glu, ratio total gliadin content/total glutenin content; Protein, total protein content in percent of total dry flour weight; Starch, total starch content in percent of total dry flour weight; SDSS, SDS sedimentation test; Weight 1000, 1000 grain weight in grams. (***, **, *, ·) Means are significantly different to control as determined by multiple comparisons at P < 0.001, P < 0.01, P < 0.05, P < 0.1 respectively.
Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us