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Table 3 Prolamins and non-gluten contents, gliadins and glutenins contents, total protein and starch contents, SDS sedimentation test and 1000 seed weight of transgenic and wild-type lines

From: Integration of promoters, inverted repeat sequences and proteomic data into a model for high silencing efficiency of coeliac disease related gliadins in bread wheat

      Gliadin (μg/mg of flour) Glutenin (μg/mg of flour) Gli/Glu (μg/mg of flour) Protein (%) Starch (%) SDSS (mL · g − 1) Weight 1000 (g)
Genotype Line Constructs Promoter iTarget ω α γ Total LMW HMW Tota Prolamins Non-gluten
BW208 Wild type Control C C 11.89 39.59 27.76 78.24 15.51 8.05 23.56 3.31 101.80 26.66 14.21 55.37 12.54 44.25
  A1152 pGhpg8.1 + pDhpg8.1 GD g 14.98 45.82 1.78*** 61.59 18.09* 9.61 27.70 2.24 89.29 45.47 14.90 54.77 13.49 46.79
  A1158 pGhpg8.1 + pDhpg8.1 GD g 22.56 56.05 2.18*** 79.78 17.61 10.01 27.62 2.83 107.40 25.28 14.67 54.83 11.88 46.86
  A1406 pGhpg8.1 + pDhpg8.1 GD g 18.19 57.84 1.67*** 76.70 14.47 11.39*** 25.86 2.94 102.56 53.06 17.17*** 51.74 9.62*** 39.84**
  C655 pDhpg8.1 D g 16.94 49.20 5.29*** 70.44 19.59*** 9.38 28.98 2.38 99.41 33.23 14.72 54.00 13.70 41.56
  C657 pDhpg8.1 D g 25.12 63.24 7.25*** 94.61 17.92* 8.88 26.80 3.51 121.41 7.75 14.27 54.48 12.39 44.21
  D445 pDhpg8.1 D g 41.54* 78.41 2.17*** 121.12 21.14*** 11.42** 32.55*** 3.73 153.68 -7.61 16.16*** 52.68 13.55 34.8***
  D577 pGhpg8.1 G g 18.50 48.90 4.72*** 71.13 18.48** 9.79 28.27 2.48 99.39 35.06 14.87 54.46 12.85 42.65
  D623 pDhpg8.1 D g 16.27 41.91 3.81*** 60.99 18.78*** 9.81 28.60 2.12* 89.58 45.63 14.92 54.66 12.39 44.50
  D682 pGhpg8.1 G g 24.02 56.85 4.14*** 84.01 19.01*** 9.72 28.72 2.95 112.74 15.59 14.20 54.77 13.02 45.36
  28A pDhp_ω/α D o 7.29*** 17.13*** 5.98*** 29.39*** 20.22*** 16.70*** 36.92*** 0.82*** 66.31** 58.41 13.81 55.21 12.56 39.45***
  28B pDhp_ω/α D o 6.39*** 13.46** 5.09*** 23.94*** 19.95*** 18.36*** 38.31*** 0.60*** 62.26*** 64.38 13.99 53.92 12.57 42.35
  D770 pGhp_ω/α G o 6.25*** 10.31*** 2.95*** 18.50*** 17.91* 19.85*** 37.76*** 0.49*** 56.27*** 78.30* 14.82 55.03 12.18 39.56**
  D783 pDhp_ω/α D o 5.05*** 10.87*** 4.21*** 19.13*** 19.6*** 17.00*** 36.6*** 0.53*** 55.73*** 73.40* 14.26 55.98 12.36 42.56
  D793 pGhp_ω/α G o 5.84*** 5.78*** 1.39*** 12.01*** 10.01*** 14.34*** 24.36 0.50*** 36.37*** 95.60*** 14.48 55.68 8.55*** 40.66*
  D894 pGhp_ω/α G o 5.24*** 9.86*** 2.87*** 16.98*** 18.74*** 16.37*** 35.11*** 0.50*** 52.10*** 78.72* 14.45 54.70 12.08 41.52
  E33 pDhpg8.1 + pDhp_ω/α D go 5.88*** 8.73*** 1.66*** 15.27*** 15.57 19.86*** 35.44*** 0.43*** 50.71*** 90.83** 15.63 54.68 11.94 42.22
  E35 pDhpg8.1 + pDhp_ω/α D go 6.47*** 10.16*** 1.75*** 17.38*** 16.45 18.45*** 34.90*** 0.50*** 52.28*** 80.44* 14.65 54.31 12.27 40.84*
  E39 pDhpg8.1 + pDhp_ω/α D go 6.55*** 14.88*** 1.99*** 22.41*** 15.47 11.60*** 27.07 0.84*** 49.49*** 93.16** 15.65 52.81 10.25*** 37.94***
  E42 pDhpg8.1 + pDhp_ω/α D go 5.51*** 9.40*** 1.29*** 15.2*** 11.66** 10.07 21.72 0.71*** 36.92*** 97.86*** 14.90 51.78 8.98*** 36.51***
  E76 pDhpg8.1 + pDhp_ω/α D go 6.38*** 3.70*** 1.05*** 10.12*** 6.72*** 13.04*** 19.76 0.53*** 29.88*** 103.69*** 14.77 53.44 7.18*** 33.88***
  E82 pDhpg8.1 + pDhp_ω/α D go 6.87*** 4.14*** 1.04*** 11.06*** 6.08*** 9.29 15.37*** 0.76*** 26.43*** 104.56*** 14.46 53.28 6.23*** 35.78***
  E83 pDhpg8.1 + pDhp_ω/α D go 6.06*** 3.15*** 1.02*** 9.24*** 4.99*** 8.47 13.46*** 0.68*** 22.70*** 115.74*** 15.28 50.91* 6.93*** 32.61***
BW2003 Wild type Control C C 12.87 28.15 22.93 62.95 13.93 10.83 24.76 2.53 87.70 39.21 14.01 57.48 11.84 44.65
  22A pGhpg8.1 G g 28.25 49.06 3.74*** 80.06 18.39*** 16.87*** 35.25*** 2.21 115.31 18.58 14.87 55.85 14.62*** 45.03
  22C pGhpg8.1 G g 20.79 39.08 2.90*** 61.76 17.61*** 14.72* 32.33** 1.89 94.10 42.64 15.15 55.68 14.11*** 46.71
  24A pGhpg8.1 G g 36.51 59.00 5.02*** 99.53 18.00** 15.06* 33.06* 3.10 132.59 55.73 16.08· 54.60 13.71** 40.62
  24B pGhpg8.1 G g 18.90 34.64 2.75*** 55.29 15.47 12.74 28.21 1.97 83.50 53.22 14.68 55.65 11.98 43.99
  C217 pDhpg8.1 D g 25.01 45.26 9.03*** 78.29 15.92 13.64 29.55 2.50 107.85 24.26 14.70 56.56 12.91 43.33
  D598 pDhpg8.1 D g 16.82 33.07 6.91*** 55.80 14.90 11.87 26.76 2.07 82.56 53.87 15.12 56.67 12.21 43.01
  D715 pGhpg8.1 G g 17.99 34.96 4.94*** 56.90 16.99** 14.34· 31.33* 1.81* 88.23 53.77 15.68 56.71 13.75*** 47.96
  D716 pGhpg8.1 G g 18.38 35.48 2.84*** 55.70 16.67** 14.62* 31.28* 1.77 86.98 40.84 14.16 54.49 14.04*** 48.63*
  D815 pGhpg8.1 G g 16.95 32.62 2.94*** 51.51 15.84 12.61 28.44 1.79 79.95 56.99 14.85 57.19 13.08 38.01***
  D874 pDhp_ω/α + pGhp_ω/α GD o 7.17*** 5.22*** 3.18*** 14.58*** 6.29*** 19.96*** 26.25 0.57*** 40.83*** 95.71* 15.06 54.5 8.95*** 39.88**
  D876 pDhp_ω/α + pGhp_ω/α GD o 6.17*** 5.36*** 3.12*** 13.65*** 5.96*** 19.87*** 25.83 0.54*** 39.48*** 95.27* 14.89 54.68 8.94*** 39.29***
  E140 pDhp_ω/α D o 6.43*** 10.49*** 6.29*** 22.21*** 14.42 19.93*** 34.35*** 0.65*** 56.56*** 76.2 14.7 56.45 12.24 41.53
  E146 pDhp_ω/α D o 8.57*** 13.20*** 8.15*** 28.92*** 13.17 21.73*** 34.90*** 0.80*** 63.81** 70.35 14.85 54.61 12.05 42.49
  E122 pDhpg8.1 + pDhp_ω/α D go 7.42*** 12.57*** 3.32*** 22.3*** 12.62 18.99*** 31.61 0.70*** 53.91*** 103.37* 17.44*** 52.19** 11.79 34.32***
  E93 pGhpg8.1 + pGhp_ω/α G go 7.03*** 7.70*** 2.15*** 15.88*** 8.52*** 15.62** 24.15 0.67*** 40.03*** 98.71** 15.33 54.64 9.38*** 38.65***
  E96 pGhpg8.1 + pGhp_ω/α G go 7.13*** 7.09*** 2.03*** 15.24*** 7.56*** 17.38*** 24.94 0.62*** 40.18*** 94.37* 14.89 54.57 9.33*** 41.62·
  1. Promoter, the promoter used to drive the silencing fragments; C, control line; G, γ-gliadin promoter; D, H-hordein promoter; GD, the γ-gliadin and D-hordein promoter; iTarget, silencing fragments; g, γ-gliadin silencing fragment; o, ω/α-gliadin silencing fragment; go, both silencing fragments; ω, ω-gliadins; α, α-gliadins; γ, γ-gliadins; total, total gliadin content; HMW, high molecular weight; LMW, low molecular weight; Gli/Glu, ratio total gliadin content/total glutenin content; Protein, total protein content in percent of total dry flour weight; Starch, total starch content in percent of total dry flour weight; SDSS, SDS sedimentation test; Weight 1000, 1000 grain weight in grams. (***, **, *, ·) Means are significantly different to control as determined by multiple comparisons at P < 0.001, P < 0.01, P < 0.05, P < 0.1 respectively.