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Table 1 Significance and percent of explained variance of the fixed and random effects of mixed model for each variable studied

From: Integration of promoters, inverted repeat sequences and proteomic data into a model for high silencing efficiency of coeliac disease related gliadins in bread wheat

  Model 1 Gliadin Glutenin  
   ω α γ Tota LMW HMW Total Gli/Glu Prolamins Non-gluten Protein Starch SDSS Weight 1000
Fixed Protein 0.66*** 0.69** 1.26** 0.55** 0.06 0.66* 0.05 0.38* 0.65* 2.68*** NA 3.35** 0.16 2.89***
iTarget 2.59*** 32.51*** 49.35*** 74.58*** 34.62*** 32.00*** 22.56*** 16.84*** 21.46*** 5.34*** 6.77*** 9.08*** 37.54*** 33.96***
Genotype 0.47** 1.02*** 5.39*** 0.01 5.25** 15.35*** 0.05 0.01 0.11 0.80* 0.95· 5.6*** 0.05 1.43*
iTarget x Genotype 0.04 1.17** 1.54* 0.73* 7.11*** 1.51* 5.12*** 0.84** 1.28* 0.58 2.20 2.17 4.12*** 0.59
Random Itarget x Year 7.89*** 1.52 0.38 0.00 0.00 0.05 0.00 5.13 2.79 11.97** 0.00 0.00 0.00 0.00
Block x Year 3.93*** 4.61*** 1.81 1.44*** 0.19 1.83*** 0.62 2.71* 7.03*** 12.82*** 11.91*** 4.46*** 0.61 0.00
Genotype x Year 0.35 0.00 0.00 0.00 1.02 0.05 3.05 2.08 0.00 0.00 0.00 0.00 4.69*** 0.00
Year 69.77** 13.46 0.00 10.86 5.96*** 17.31 0.67 30.3* 16.73 4.32 0.00 6.48 3.03 3.20
Residual 14.31 45.03 40.27 11.82 45.79 31.25 67.89 41.70 49.94 61.49 78.16 68.85 49.80 57.94
  Model 2               
Fixed Protein 0.46 0.54 0.01 0.44 0.47 0.12 0.26 0.49 0.49 73.44*** NA 0.71 0.03 0.41
iTarget 4.99*** 4.63*** 6.04*** 3.47*** 35.99*** 30.54*** 14.55*** 3.30*** 3.75*** 0.72*** 3.66* 5.22** 39.65*** 29.29***
Promoter 0.59 0.32 11.13*** 0.68 12.67*** 0.34 5.22*** 0.35 1.13* 0.22* 2.05 1.26 9.08*** 2.38*
Random Promoter x Year 0.00 0.00 0.25 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
iTarget x Year 7.67 14.32*** 2.69 15.55*** 0.00 0.00 0.00 20.17*** 11.78** 3.3** 0.00 0.00 0.00 0.00
Block x Year 17.32*** 20.11*** 13.14*** 19.59*** 5.27*** 22.03*** 20.89*** 9.16*** 27.37*** 7.61*** 1.07 6.8** 3.94** 1.98
Year 0.00 0.00 0.00 0.00 0.17 0.00 0.00 7.67 0.00 0.00 9.13** 0.00 0.00 0.82
Residual 68.98 60.07 66.75 60.26 45.43 46.96 59.08 58.86 55.49 14.72 84.10 86.02 47.30 65.12
  1. ω, ω-gliadins; α, α-gliadins; γ, γ-gliadins; total, total gliadin content; HMW, high molecular weight; LMW, low molecular weight; Gli/Glu, ratio total gliadin content/total glutenin content; Protein, total protein content in percent of total dry flour weight; Starch, total starch content in percent of total dry flour weight; SDSS, SDS sedimentation test; Weight 1000, 1000 seed weight in grams; iTarget, silencing fragments; C, control line; g, γ-gliadin silencing fragment; o, ω/α-gliadin silencing fragment; go, both silencing fragments; Promoter, the promoter used to drive the silencing fragments; G, γ-gliadin promoter; D, H-hordein promoter; GD, the γ-gliadin and D-hordein promoter. Protein was analized like variable using the Model 1 and 2 removing the covariate. ***, **, *, ·, percent of explained variances are significantly at P < 0.001, P < 0.01, P < 0.05, P < 0.1 respectively.