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Table 1 Significance and percent of explained variance of the fixed and random effects of mixed model for each variable studied

From: Integration of promoters, inverted repeat sequences and proteomic data into a model for high silencing efficiency of coeliac disease related gliadins in bread wheat

 

Model 1

Gliadin

Glutenin

 
  

ω

α

γ

Tota

LMW

HMW

Total

Gli/Glu

Prolamins

Non-gluten

Protein

Starch

SDSS

Weight 1000

Fixed

Protein

0.66***

0.69**

1.26**

0.55**

0.06

0.66*

0.05

0.38*

0.65*

2.68***

NA

3.35**

0.16

2.89***

iTarget

2.59***

32.51***

49.35***

74.58***

34.62***

32.00***

22.56***

16.84***

21.46***

5.34***

6.77***

9.08***

37.54***

33.96***

Genotype

0.47**

1.02***

5.39***

0.01

5.25**

15.35***

0.05

0.01

0.11

0.80*

0.95·

5.6***

0.05

1.43*

iTarget x Genotype

0.04

1.17**

1.54*

0.73*

7.11***

1.51*

5.12***

0.84**

1.28*

0.58

2.20

2.17

4.12***

0.59

Random

Itarget x Year

7.89***

1.52

0.38

0.00

0.00

0.05

0.00

5.13

2.79

11.97**

0.00

0.00

0.00

0.00

Block x Year

3.93***

4.61***

1.81

1.44***

0.19

1.83***

0.62

2.71*

7.03***

12.82***

11.91***

4.46***

0.61

0.00

Genotype x Year

0.35

0.00

0.00

0.00

1.02

0.05

3.05

2.08

0.00

0.00

0.00

0.00

4.69***

0.00

Year

69.77**

13.46

0.00

10.86

5.96***

17.31

0.67

30.3*

16.73

4.32

0.00

6.48

3.03

3.20

Residual

14.31

45.03

40.27

11.82

45.79

31.25

67.89

41.70

49.94

61.49

78.16

68.85

49.80

57.94

 

Model 2

              

Fixed

Protein

0.46

0.54

0.01

0.44

0.47

0.12

0.26

0.49

0.49

73.44***

NA

0.71

0.03

0.41

iTarget

4.99***

4.63***

6.04***

3.47***

35.99***

30.54***

14.55***

3.30***

3.75***

0.72***

3.66*

5.22**

39.65***

29.29***

Promoter

0.59

0.32

11.13***

0.68

12.67***

0.34

5.22***

0.35

1.13*

0.22*

2.05

1.26

9.08***

2.38*

Random

Promoter x Year

0.00

0.00

0.25

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

iTarget x Year

7.67

14.32***

2.69

15.55***

0.00

0.00

0.00

20.17***

11.78**

3.3**

0.00

0.00

0.00

0.00

Block x Year

17.32***

20.11***

13.14***

19.59***

5.27***

22.03***

20.89***

9.16***

27.37***

7.61***

1.07

6.8**

3.94**

1.98

Year

0.00

0.00

0.00

0.00

0.17

0.00

0.00

7.67

0.00

0.00

9.13**

0.00

0.00

0.82

Residual

68.98

60.07

66.75

60.26

45.43

46.96

59.08

58.86

55.49

14.72

84.10

86.02

47.30

65.12

  1. ω, ω-gliadins; α, α-gliadins; γ, γ-gliadins; total, total gliadin content; HMW, high molecular weight; LMW, low molecular weight; Gli/Glu, ratio total gliadin content/total glutenin content; Protein, total protein content in percent of total dry flour weight; Starch, total starch content in percent of total dry flour weight; SDSS, SDS sedimentation test; Weight 1000, 1000 seed weight in grams; iTarget, silencing fragments; C, control line; g, γ-gliadin silencing fragment; o, ω/α-gliadin silencing fragment; go, both silencing fragments; Promoter, the promoter used to drive the silencing fragments; G, γ-gliadin promoter; D, H-hordein promoter; GD, the γ-gliadin and D-hordein promoter. Protein was analized like variable using the Model 1 and 2 removing the covariate. ***, **, *, ·, percent of explained variances are significantly at P < 0.001, P < 0.01, P < 0.05, P < 0.1 respectively.
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BMC Plant Biology

ISSN: 1471-2229

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