Figure 2
From: The effect of Translationally Controlled Tumour Protein (TCTP) on programmed cell death in plants
Effect of AtTCTP on tunicamycin induced cell death. Cell death quantification of tobacco leaf discs transiently over-expressing TCTP of Arabidopsis thaliana (AtTCTP). A shows ion leakage in response to 2.5 μg · ml-1 tunicamycin, calculated relative to time point T0. Statistical significances were calculated using analysis of variance (P < 0.05; a indicates wildtype (wt) vs. AtTCTP over-expression). The panel on the left side of B displays tobacco leaf discs floated on 2.5 μg · ml-1 tunicamycin for 69 hours, either over-expressing AtTCTP or wildtype tobacco (wt). The panel on the right side shows trypan blue staining of dead cells of leaf discs. C shows western blot analysis of leaf discs expressing AtTCTP over time (2, 21, 27, 45, 54, and 69 hours of disc floating).