The effect of humidity on resistance of A. thaliana Col0 to B. cinerea and ROS accumulation after SMS. Leaves were stimulated by SMS and maintained for 1.5 h under high humidity in tightly covered well-watered trays (humid) or left in uncovered trays (dry) at room conditions prior to inoculation with B. cinerea and ROS detection. (A) Resistance to B. cinerea (n = 64; ±SE). One representative picture of the growth of B. cinerea was placed above each histogram as a visual illustration (trypan blue staining was carried out 3 days after inoculation). (B) Quantification of ROS production; three representative images of the fluorescent leaf surface were placed above each histogram as a visual illustration (n = 16; ±SD). Asterisks indicate statistically significant differences between treated samples and non-treated samples in humid and dry conditions, T-Test (p < 0,01).