Figure 5

OsNAP RNAi transgenic plants displayed delay of leaf senescence phenotype. A, OsNAP RNAi transgenic lines (RNAi-1 and RNAi-2) and WT plants at 50 DAH. B, flag leaves of RNAi-1, RNAi-2 and WT at 50 DAH. C, Second top leaves of RNAi-1, RNAi-2 and WT at 50 DAH. D, Content of chlorophyll of flag and second top leaves in RNAi-1, RNAi-2 and WT plants at 50 DAH. Values are means ± SD (n = 3). **P < 0.01 (t test). E, qRT-PCR analysis of the expression levels of OsNAP and OsDOS in flag leaves of RNAi-1, RNAi-2 and WT plants at 50 DAH. Each value represents the mean ± SD evaluated from three replicates.