Expression pattern of OsNAP . A, qRT-PCR analysis of OsNAP transcript levels in flag leaves of different development periods in rice. SL1, flag leaves at the booting stage, SL2, flag leaves at the anthesis stage, SL3, flag leaves at the mature stage, SL4, flag leaves at the ripening stage. B, Tissue localization of OsNAP. a, callus; b, young leaf; c, mature leaf; d, senescent leaf; e, sheath; f, node and internode; g, mature seed. C, qRT-PCR analysis of OsNAP transcripts with MeJA treatment at different time points. Roots of Zhonghua 11 plants at the trefoil stage were immersed in nutrition solutions contained 0.1 mM MeJA, and several plants were collected at different points after treatment for RNA isolation.