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Figure 1 | BMC Plant Biology

Figure 1

From: Identification and functional characterization of a rice NAC gene involved in the regulation of leaf senescence

Figure 1

Subcellular localization of OsNAP. A, Nuclear localization of OsNAP in onion cells. OsNAP cDNA was fused to GFP under control of the maize (Zea mays) ubiquitin promoter and the construct was expressed in onion cells. (a) The transfected cells expressing GFP were photographed; (b) DAPI stained; (c) Bright field. (d) and (e), GFP alone. The bar indicates 80 μm. B, Nuclear localization of OsNAP in Arabidopsis protoplast. Arabidopsis cell protoplasts were transformed using 35S::OsNAP-GFP and 35S::Ghd7-CFP. Fluorescence signals (GFP and CFP) were detected by a laser confocal-scanning microscope. (a), 35S::Ghd7-CFP; (b), 35S::OsNAP-GFP; (c), Bright field; (d), overlap images of (a), (b) and (c). (e), (f) indicated autofluorescence emitted by chloroplasts; (g), Bright field; (h), overlap images of (e), (f) and (g). The bar indicates 10 μm.

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