Figure 6

Green fluorescent protein expression in Aristolochiaceae. A, C, E, G, I . Light images. B, D, F, H, J. Fluorescent images. A-D. Leaf explants 10 days after Agrobacterium tumefaciens infection A, B. Saruma henryi C, D. A. fimbriata E, F. Regenerating A. fimbriata stem explant. G, H, I, J. Regenerating A. fimbriata roots (one root is shaded from light source in G). K. Gel image of PCR products; Lane L- 200bp ladder, bright band at 1K bp with corresponding bands at each 200bp; Lane 2- A. fimbriata (WT) DNA; Lane 3- In vitro transformed callus 1; Lane 4- In vitro transformed callus 2; Lane 5- In vitro transformed callus; Lane 6- Negative Control; Lane 7-Plasmid PC (1 ng/ul).