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Table 3 Three-factor ANOVA [F df (5,48) ] values of all metabolites in Experiment 3

From: Tall fescue endophyte effects on tolerance to water-deficit stress

Metabolite

Endophyte

Day

Stress

Day * Endophyte

Stress * Endophyte

Stress * Day

Endophyte * Stress * Day

Shoot glucose

0.55

15.38 ***

188.58***

1.7

0.18

30.31***

2.67*

Shoot fructose

5.01*

24.51***

344.35***

1.49

11.72

34.71***

1.56

Shoot sucrose

4.78*

26.49***

97.38***

0.39

5.94*

17.83***

2.98*

Shoot GFS

3.27

24.76***

320.43***

1.15

7.24**

35.35***

2.01

Shoot proline

0.5

37.81***

329.02***

0.87

0.6

38.41***

0.81

Shoot glutamine

0.01

9.91 ***

117.18***

1.94

0.02

12.05***

2.62*

Shoot glutamic acid

3.83

7.29***

0.66

3.64**

1.97

6.94***

2.67*

Shoot asparagine

0.82

8.43***

30.99***

0.41

0.12

3.87**

1.13

Shoot aspartic acid

0.76

1.17

33.33***

1.37

0.33

3.33*

0.52

Shoot tryptophan

5.01*

24.51***

344.35***

1.49

11.72**

34.71***

1.56

Shoot phenyl alanine

4.78*

26.49***

97.38***

0.39

5.94*

17.83***

2.98*

Shoot tyrosine

3.27

24.76***

320.43***

1.15

7.24**

35.35***

2.01

Shoot threonine

0.55

15.38***

188.58***

1.7

0.18

30.31***

2.67*

Shoot trehalose

23.82***

10.87***

69.51***

2.83*

11.06**

16.26***

5.79***

Shoot lolines

507.88***

2.35

18.51***

2.35

18.51***

1.23

1.23

Root glucose

0

3.41*

2.54

0.95

0.55

4.62**

1.87

Root fructose

2.2

8.00 ***

103.20***

0.93

1.56

14.24***

2.75*

Root sucrose

4.23*

13.00***

32.14***

2.05

1.98

9.98***

1.27

Root GFS

3.22

11.21***

72.15***

1.43

1.44

13.96***

2.51*

Root proline

0

9.55***

59.35***

1.93

0

8.94***

1.67

Root glutamine

1.95

3.22*

24.14***

2.06

0.73

2.18

1.55

Root glutamic acid

0.38

12.94***

6.74*

0.56

0.05

0.56

0.54

Root asparagine

1.11

2.39

4.28*

1.21

1.61

0.4

1.53

Root aspartic acid

0.17

32.52***

18.15***

0.47

0.08

3.75**

0.34

Root tryptophan

0.25

5.62***

56.82***

1

0.03

7.29***

0.46

Root phenylalanine

0.31

5.38***

54.78***

1.13

0.03

7.01***

0.91

Root tyrosine

0.42

1.35

13.37***

0.9

0.13

3.97**

0.77

Root threonine

2.07

7.32***

84.63***

1.13

2.75

9.03***

1.37

Root lolines

455.13***

9.26***

19.28***

9.26***

19.28***

0.93

0.93

  1. ‘*’ denotes p-values = > 0.01 - 0.05; ‘**’ denotes p-values = > 0.001 - 0.01; ‘***’ denotes p-values = < 0.001.