PCR analysis of SlAGO genes in tomato. (A) Expression patterns of SlAGO genes in different organs. R, S, L, F stands for roots, stems, leaves and flowers collected from flowering tomato. (B) Expression patterns of SlAGO genes during bud development. Buds were divided in to 5 developmental stages from stage 1 to stage 5 according to the length of bud. Stage 1 stands for no more than 1 mm; Stage 2 stands for between 2 to 3 mm, stage 3 stands for between 4 to 5 mm, stage 4 stand for between 6 to 7 mm and stage 5 stands for between 8 to 9 mm. (C) Expression patterns of AGO genes in different organs of tomato flowers. Stamens, pistil, petals and sepals were collected from flowering plants. (D) Expression patterns of tomato AGO genes during stamen development. -2D, 0D and 4D stands for stamens collected from flowers 2 days before anthesis, the very day of anthesis and 4 days post anthesis respectively. (E) Expression patterns of SlAGO genes during tomato fruit development. -2D stands for fruits collected 2 days before anthesis; 0D, 4D, 8D 16D and 32D stands for fruits collected on the 0, 4, 8, 16 and 32 day post anthesis respectively; YF stands for yellow fruits and RF stands for red fruits. Ubi3 was used as a reference gene to normalize expression level and 30 cycles of PCR were performed for each primer.