Table 1 PCR primers used for generating Puroindoline-D1 alleles and primer walking
Primer name | Forward primer | Reverse primer | Tma | Expected PCR size |
|---|---|---|---|---|
Pinb-D1b1 | ATGAAGGCCCTCTTCCTCA | CTCATGCTCACAGCCGCT | 58°С | 250 bp |
Pinb-D1b2 | ATGAAGGCCCTCTTCCTCA | CTCATGCTCACAGCCGCC | 58°С | 250 bp |
Pina-D1 | CATCTATTCATCTCCACCTGC | GTGACAGTTTATTAGCTAGTC | 58°С | 524 bp |
Pinb-D1 | GAGCCTCAACCCATCTATTCATC | CAAGGGTGATTTTATTCATAG | 58°С | 597 bp |
Pina-N1 | AATACCACATGGTTCTAGATACTG | GCAATACAAAGGACCTCTAGATT | 60°С | 776 bp |
Pina-N2 | TCAACATTCGTGCATCATCA | CTTCATTCGTCAGAGTTCCAT | 60°С | 436 bp |
Pina-N3 | CATCTATTCATCTCCACCTGC | CACTATATTGCCGGGATTTT | 58°С | 440 bp |
Pina-N4 | AGTGGTCTGATGGAAGCGT | TGGAAAAAACTAGGTTGGGA | 54°С | 546 |
BsrDI_Pina-D1n | TCACCTGGCGTTGGTGGCAAT | CGGCAGGTTCTTGGCTTCTTGTAT | 66°С | 197 |
BalI_Pina-D1l | GAGTGTTGCAGTCGGCTTGG | GGCAGGTTCTTGGCTTCTTGT | 61°С | 143 |
Pina-1 | TACCTGTAGCCCCCAAGTTT | GAGTCGCTGCAGGCTTACG | 58°С | 677 bp |
Pina-2 | AATGCCCAACCTATAACCCG | CCATAGCCATGCCTCTTGAT | 57°С | 647 bp |
Pina-3 | AACCAGACCGGGCTGATAGT | CCAAGACGATGGAGGGAAAG | 62°С | 889 bp |
Pina-4 | GATAACCCTAATCCGGAGTAA | CAAATCTTGCCAGTTTCAGC | 54°С | 1036 bp |
Pina-5 | ATGGCAACAGGTCCTCTTCG | ATAGCTCAATGGGCAGGCAC | 60°С | 579 bp |
Pina-6 | TGGAAGGTTGAGGTGACTGC | TTTTTATTGGCGTTCGACTG | 57°С | 512 bp |
Pina-7 | GCAGACGGCGGTTGATAGTA | GTGAGAAGAGGGCGAGGGAA | 62°С | 720 bp |
Pina-8 | GGTGGGCGTCAACTGTCGTG | ATGCTACCTTGCTTTGTCCTCC | 62°С | 606 bp |
Pina-9 | TGACCAGGGAGCGAATACG | TGACGAGTTTCACGCTTCCA | 59°С | 1143 bp |
Pina-10 | ATAATAGCACCGGAACGCA | AGTCTTTCTCTCAGCATACACG | 54°С | 1022 bp |
Pina-11 | GACCCGCATATAAGAAAACCGAT | TCCCACATGTTAGTGTCTGCAAAG | 61°С | 760 bp |
Pina-12 | CTTTTATGGCATTGTACATGGGGAG | TGTCAGTGTGTTTTGGTGCAGGTGG | 65°С | 950 bp |
Pina-13 | AGAAACATTTGACATGAACGAC | AGATGAGATGAGGCCACACC | 54°С | 751 bp |
Pina-14 | GTATGTTCTTGGTGGTGGTTT | TGGTCTGGGAGGATGAATAG | 54°С | 687 bp |
Pina-15 | CAGGACGAAATACGTTGAAA | TGGAAAAAACTAGGTTGGGA | 54°С | 1004 bp |
Pina-16 | AGAGAGTGCCCCAAAAGGTA | GGAAATCCCTTTGGTCAATG | 56°С | 623 bp |
Pina-17 | TGACCAAAGGGATTTCCGTA | AAGATGGAGATGATGATGCC | 54°С | 511 bp |
Pina-18 | CTTCAAGTTCCTCCAGACCT | CACTATATTGCCGGGATTTT | 54°С | 484 bp |
Pina-19 | TGTTGTCTGAGTCTTCCTGTTT | GCTCTGGTGGTTCGACTTCT | 54°С | 624 bp |
Pina-20 | TGAGAGGCCTTGACATTTCC | AACGATGTGTGTCAGGCGGT | 57°С | 962 bp |
Pina-21 | CTCGCCTTCTTCTTTTGTCTCG | CCAACGGACTCATCGGCTCA | 61°С | 890 bp |
Pina-22 | CGATGAGTCCGTTGGAGGTA | GAAGATGGCCTTTTCGATGC | 58°С | 906 bp |
Pina-23 | GGGTTTTTCTCTCAACTGGG | GAACAGTTTTTCACAATGGG | 54°С | 1114 bp |
Pina-24 | AGTAAACAGGCACTCTCGCT | CTGGTTTTTCCCTGTTTCAT | 54°С | 985 bp |
Pina-25 | CAGGGAAAAACCAGCAAAAC | GCAGGAAGTATGTCAAGCGT | 55°С | 1041 bp |
Pinb-1 | AGCGGGGTACTAGACAACAG | GATAAATACATACACCTGTCGTTC | 53°С | 950 bp |
Pinb-2 | CAAAAGCGACGGGCACAGAG | CTCTACTTTCCGGATGTTGCGA | 62°С | 757 bp |
Pinb-3 | ATCCTCTCCTTGTCACCCTG | GCTCACGCTTTAAGCTTTTG | 56°С | 527 bp |
Pinb-4 | CAGAAAACCACGGCTAGAAG | GGACATTGTTGAGAACCACCT | 55 | 529 |
Pinb-5 | CTGCGGAAAAAAAAATCTGG | CTAACATCTAAAGCCGGAGG | 55 | 1531 |
Pinb-6 | AGTGCGTCAGACCGGTTTGT | GGTGGTGGTGATTGGTGAAG | 58 | 1129 |
Pinb-7 | GGAAATTGTGTCGCCTCATC | AAAGCCGCATCTTCTTGTAG | 55 | 942 |
Pinb-8 | CAGAGACGTGTTTATGGGAG | GCCCTTGTTGTCTTCTTTTA | 52 | 925 |
Pinb-9 | TTACTCGAAGGACTCGGAAG | AGAGATAGTGTTGGCATGGA | 52 | 1201 |
Pinb-10 | TGCCAACACTATCTCTGCCTC | ATGATCCCGTGACTAACTCCT | 55 | 1115 |
Pinb-11 | ACGGGTATCTCTGAAAGTGTC | TAAGCGTACGTGTAAGGTCG | 53 | 1458 |
Pinb-12 | AGTCTTATCTTGTTTCGGCG | CTCATATGCTCCATGTTTCTC | 52 | 1501 |
Pinb-13 | TGTTCGAGGAGCTGAAAATG | TGGTTCGCACGTGTCAAAT | 56 | 1126 |