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Figure 2 | BMC Plant Biology

Figure 2

From: Interaction between two rice mitogen activated protein kinases and its possible role in plant defense

Figure 2

Specificity of interaction between OsMPK20-4 and OsMPK3. (A) Reporter yeast strain AH109 was co-transformed with the recombinant OsMPK20-4 in pGBKT7 and close relatives of OsMPK3 including OsMPK4 and OsMPK6 in pGADT7. (B) OsMPK3 in pGBKT7 was transformed into AH109 along with close relatives of OsMPK20-4 including OsMPK17-1 and OsMPK20-2. Transformants were selected on SD [-leu-trp] DDO media and the interaction was checked on SD [-trp-leu-ade-his] QDO media. (C) Full length OsMPK20-4 is required for efficient interaction with OsMPK3. AH109 yeast reporter strain was co-transformed with deletion constructs of OsMPK20-4, OsMPK20-4KD containing first 358 amino acids and OsMPK20-4CT containing the long C-terminal region of 211 amino acids in both AD and BD vectors. Transformants were selected on DDO and the interaction was checked on QDO. AH109 co-transformed with SV40 large T antigen in pGADT7 vector and p53 in pGBKT7 vector served as positive control in all the experiments.

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