Skip to main content

Table 5 Shoot regeneration from cotyledons (C) and hypocotyls (H) of L. campestre cultured on the media containing either 0.5 or 1.0 mg L -1 2, 4-D (2, 4-D 0, 5 and 2, 4-D1.0) for 2 or 3 days (2d and 3d), followed by transfer to 1.0 mg L -1 zeatin (z1.0) for 2 or 6 weeks (2w and 3w) before being transferred to 2.0 mg L -1 zeatin (z 2.0)*

From: Development of an efficient regeneration and transformation method for the new potential oilseed crop Lepidium campestre

Treatment Shoot regeneration %**
  Means ± SD
2,4-D 0,5, 3d→z 1.0, 2w→z 2.0, C 4,7 ± 0.21 c
2,4-D 0,5, 3d→z 1.0, 2w→z 2.0, H 7.2 ± 0.26 c
2,4-D 0,5, 2d →z 1.0, 6w→z 2.0, C 1.0 ± 0.10 d
2,4-D 0,5, 2d →z 1.0, 6w→z 2.0, H 67.0 ± 0.47 a
2,4-D 1.0, 3d→z 1.0, 2w→z 2.0, C 3.3 ± 0.18 c
2,4-D 1.0, 3d→z 1.0, 2w→z 2.0, H 0.0 ± 0.00 d
2,4-D 1.0, 2d →z 1.0, 6w→z 2.0, C 1.0 ± 0.10 d
2,4-D 1.0, 2d →z 1.0, 6w→z 2.0, H 29.8 ± 0.45 b
  1. *Each treatment contained 30 explants and repeated at least twice and the data are means ±SD. **Means followed by different letters indicate significant differences at p = 0.05.