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Table 3 Shoot regeneration from cotyledons (C) and hypocotyls (H) of L. campestre cultured on the media containing either 0.5 or 1.0 mg L -1 2,4-D (2,4-D 0.5 or 2,4-D1.0) for 3 days (3d), followed by transfer to the medium containing either 1.0 mg L -1 zeatin (z1.0), or 1.1 mg L -1 TDZ (TDZ1.1)*

From: Development of an efficient regeneration and transformation method for the new potential oilseed crop Lepidium campestre

Treatment

Shoot regeneration %**

 

Means ± SD

2,4-D 0,5, 3d→z 1.0, C

0.0 ± 0.00 d

2,4-D 0,5, 3d→z 1.0, H

0.0 ± 0.00 d

2,4-D 1.0, 3d→z 1.0, C

5.0 ± 0.22 c

2,4-D 1.0, 3d→z 1.0, H

2.7 ± 0.16 c

2,4-D 0,5, 3d→TDZ 1.1, C

0.0 ± 0.00 d

2,4-D 0,5, 3d→TDZ 1.1, H

92.7 ± 0.26 a

2,4-D 1.0, 3d→TDZ 1.1, C

0.0 ± 0.00 d

2,4-D 1.0, 3d→TDZ 1.1, H

27.5 ± 0.45 b

  1. *Each treatment contained 30 explants and repeated at least twice and the data are means ±SD. **Means followed by different letters indicate significant differences at p = 0.05.
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BMC Plant Biology

ISSN: 1471-2229

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