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Table 1 Shoot regeneration from cotyledons (C) and hypocotyls (H) of L. campestre grown on the media containing different plant growth regulators*

From: Development of an efficient regeneration and transformation method for the new potential oilseed crop Lepidium campestre

Treatment **

Shoot regeneration %***

 

Means ± SD

Zeatin 1.0 + NAA 0.5, C

0.0 ± 0.00 e

Zeatin 1.0 + NAA 0.5, H

0.0 ± 0.00 e

Zeatin 2.0 + NAA 0.5, C

0.0 ± 0.00 e

Zeatin 2.0 + NAA 0.5, H

0.0 ± 0.00 e

Zeatin 5.0 + NAA 0.5, C

0.0 ± 0.00 e

Zeatin 5.0 + NAA 0.5, H

0.0 ± 0.00 e

TDZ 0.44 + NAA 0.2, C

0.0 ± 0.00 e

TDZ 0.44 + NAA 0.2, H

10.0 ± 0.30 d

TDZ 1.1 + NAA 0.2, H

0.0 ± 0.00 e

TDZ 2.2 + NAA 0.2, C

0.0 ± 0.00 e

TDZ 2.2 + NAA 0.2, H

0.0 ± 0.00 e

Zeatin 5.0→TDZ 5.0, H

0.0 ± 0.00 e

2,4-D 0.5, 2 days→zeatin 2.0, H

48.3 ± 0.50 b

2,4-D 1.0, 2 days→zeatin 2.0, H

28.6 ± 0.45 c

2,4-D 1.0, 2 days→TDZ 1.1, H

76.5 ± 0.46 a

  1. *Each treatment contained 30 explants and repeated at least twice and the data are means ±SD. **Figure after each PGR indicates the concentration in mg L-1. ***Means followed by different letters indicate significant differences at p = 0.05.