Figure 3

Expression patterns of the nine ZmZIP genes in maize seedlings under various metal conditions. Two-week old shoots (S) and roots (R) of maize seedlings, under standard nutrient condition (CK), Zn, Fe-deficiency (Zn-), (Fe-), 200 μM ZnSO₄ (Zn++) and 500 μM FeSO₄ (Fe++) treated, were harvested respectively at 0 h, 6 h, 12 h, 24 h, 48 h, 96 h after treatment. Relative mRNA abundance of each gene was normalized with ZmActin1 gene. Data from real-time RT-PCR experiments were analyzed according to the 2^-∆∆Ct method. The error bars indicate standard deviations.