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Table 2 Tuber proteins differentially expressed in genotype pool CIS-t versus pool CIS-s during 12 weeks storage at 4°C

From: Novel candidate genes influencing natural variation in potato tuber cold sweetening identified by comparative proteomics and association mapping

Spot

Protein

Accession no.

Ratio of mean spot volume1

T0

2w

4w

12w

Protease inhibitors

2

Serine protease inhibitor 7

P30941.2

0,24 **

0,19 **

0,28 **

0,28 **

4

Serine protease inhibitor 7

P30941.2

0,28 **

0,23 **

0,22 *

0,16 *

3

Kunitz-type enzyme inhibitor S9C11

AAL67830.1

0,37 *

0,43 **

0,49 **

0,6 *

6

Putative miraculin

CAC40756.1

0,58 *

0,53 **

0,62 **

0,42 *

14

Aspartic protease inhibitor 5

P58519.1

0,69

0,37 *

n.d.

0,65 *

10

Kunitz-type proteinase inhibitor

AAM21645.1

0,77

0,44 **

0,71 *

0,23 *

11

Kunitz-type proteinase inhibitor

AAM21645.1

0,74

n.d.

0,24 ***

n.d.

12

Kunitz-type proteinase inhibitor

AAM21645.1

0,48

0,51 *

0,52 **

0,43 **

19

Putative miraculin

CAC40756.1

3,64 *

5,02 **

2,85 **

4,34 **

20

Cysteine protease inhibitor 1

P20347.3

n.d.

4,21 ***

2,81 ***

3,32 **

21

Proteinase inhibitor II

CAA27730

1,54

2,89 **

1,62 *

0,99

22

Proteinase inhibitor II

CAA27730

1,48

2,09 *

1,45 *

1,59 **

24

Proteinase inhibitor II

CAA27730

2,89 *

1,56 *

1,25 *

1,59 *

Storage proteins

1

Patatin-2-Kuras 4

Q3YJT0

0,43 *

0,39 *

0,55 ***

0,47 **

13

Patatin Group M-3

Q2MY51

0,24

0,38 **

0,63 *

0,40 *

44

Patatin-3-Kuras 1

Q3YJS9.1

2,57 *

3,37 *

3,22 *

2,04

45

Patatin-3-Kuras 1

Q3YJS9.1

2,99 *

2,67 *

3,20 *

2,60 *

46

Patatin-3-Kuras 1

Q3YJS9.1

1,51

2,26

2,07 *

1,97

Lipid metabolism

31

Lipoxygenase

X95512.1

1,42 **

1,56 **

1,94 **

2,17 **

32

Lipoxygenase

X95512.1

1,56 *

2,02 **

1,65 **

1,79 *

33

Lipoxygenase

X95512.1

1,52

2,78 **

1,73 **

1,59 *

34

Lipoxygenase

X95512.1

1,66

3,71 *

1,72 **

1,51 *

35

Lipoxygenase

X95512.1

1,45

3,90 *

1,71 *

1,65

36

Phospholipase A1

ABQ95989.1

3,20 ***

2,82 ***

7,45 **

2,92 *

39

Phospholipase A1

ABQ95989.1

4,22 ***

2,59 ***

3,52 **

2,76 **

40

Phospholipase A1

ABQ95989.1

3,65 **

2,57 **

1,59 **

2,27 *

Glycolysis

43

Phosphoglycerate kinase

ABB87110

0,79

2,29 *

n.d.

n.d.

Heat shock proteins

5

Chloroplast small heat shock protein class I

AAQ19680.1

0,24 **

0,23 **

0,25 *

0,27 *

27

Heat shock protein (HSP70)

XP_002512741

0,87

0,40 *

n.d.

0,68 *

28

101 kDa heat shock protein

AAC83688.2

0,85

1,13

0,48 **

0,98

Proteases

47

Leucine aminopeptidase, chloroplastic

P31427.2

2,35 *

2,16 **

1,47 *

n.d.

48

Leucine aminopeptidase, chloroplastic

P31427.2

1,82 *

1,19

3,06 **

2,16 *

49

Leucine aminopeptidase, chloroplastic

P31427.2

1,43

1,78 **

2,22 **

1,66

50

Leucine aminopeptidase, chloroplastic

P31427.2

n.d.

n.d.

2,16 **

1,26

Pathogenesis related proteins

17

Putative PR-10 type pathogenesis-related protein

BAJ25784.1

0,39 *

0,75

0,93

0,79

Cytoskeleton

30

Actin

BAK57343.1

0,47 **

1,06

0,72 *

1,20

Unidentified proteins

7

Not identified

 

0,40 **

0,57 **

0,57 **

0,52 **

8

Not identified

 

0,60 *

0,44 *

0,39 *

0,22 *

9

Not identified

 

0,26**

0,14**

n.d.

n.d.

15

Not identified

 

0,46

0,46 *

0,29 *

0,44 *

16

Not identified

 

n.d.

0,12 ***

0,09 ***

0,26 ***

18

Not identified

 

3,6 **

1,73 *

1,63*

1,45

23

Not identified

 

3,64 *

1,62

2,04 *

3,12 **

25

Not identified

 

2,20 **

n.d.

2,80 **

n.d.

26

Not identified

 

1,72

n.d.

2,64 ***

2,69 *

29

Not identified

 

0,99

0,73 *

0,77

0,48 **

37

Not identified

 

3,96 **

4,25 **

4,90 **

3,96 **

38

Not identified

 

3,06 *

2,85 ***

3,88 ***

2,15

41

Not identified

 

2,52

1,62 *

2,06 **

2,54 *

42

Not identified

 

1,68 *

2,99 **

2,78 **

n.d.

  1. 1Ratio of the mean spot volume of the CIS-t pool to the mean spot volume of the CIS-s pool. Mean spot volume was calculated from three biological replicates. The level of significance is indicated by * for 0.05 > p >0.01, ** for 0.01 > p >0.001 and *** for p <0.001.
  2. n.d. spot not detectable in 2D protein profiles.
  3. 2w, 4w and 12w corresponds to weeks of storage at 4°C.