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Table 1 The operations outline both the flight and ground control experiments

From: Organ-specific remodeling of the Arabidopsis transcriptome in response to spaceflight

Operation Flight environment/duration Ground control environment/duration
Dormant plates prepared for Turn Over to Shuttle operations KSC laboratory (22 – 25 C) KSC laboratory (22 – 25 C)
Plates stowed in Nomex transport bag KSC laboratory (22 – 25 C)/2 days KSC laboratory (22 – 25 C)/2 days
Launch to ISS and loiter on station before integration into ABRS and germination Shuttle middeck locker then ISS EXPRESS Rack (22 – 25 C)/3–25 days OES Shuttle middeck locker/3–25 days on at least 24 hour delay from flight
Active growth period each plate ISS ABRS unit 22–24 C/12–14 days OES ABRS unit 22–24 C/12–14 days on at least 24 hour delay from flight
Photography and harvest of each plate to KFT ISS work areas (22–24 C)/1–2 hrs OES work areas (22–24 C)/1–2 hrs on at least 24 hour delay from flight
RNAlater soak in KFT ISS work areas (22–24 C)/12–24 hrs OES work areas (22–24 C)/12–24 hrs on at least 24 hour delay from flight
KFT Stowage on ISS MELFI (between −35 and −95 C)/8–32 days cryo freezer at KSC (−80 C)/8–32 days on at least 24 hour delay from flight
Return to KSC laboratory Double Cold Bag (−32 C)/2–3 days cryo freezer at KSC (−80)/2–3 days
Storage at KSC cryo freezer at KSC (−80)/2–7 days cryo freezer at KSC (−80)/2–7 days
Transition to PIs’ laboratory Transport on dry ice then to cryo freezer (−80) Transport on dry ice then to cryo freezer (−80)
  1. Each flight experiment was paired with a ground control that was run in parallel, on the ground, having been informed by environmental and operations data from the ISS on at least a 24 hour delay. In this manner, each flight operation was precisely replicated in the ground controls. Although there was variability in the durations of the dormancy period and the cold storage periods among the runs, for each individual experiment, the conditions were precisely replicated between each flight and ground control experiment pair.