Table 1 The operations outline both the flight and ground control experiments
From: Organ-specific remodeling of the Arabidopsis transcriptome in response to spaceflight
Operation | Flight environment/duration | Ground control environment/duration |
|---|---|---|
Dormant plates prepared for Turn Over to Shuttle operations | KSC laboratory (22 – 25 C) | KSC laboratory (22 – 25 C) |
Plates stowed in Nomex transport bag | KSC laboratory (22 – 25 C)/2 days | KSC laboratory (22 – 25 C)/2 days |
Launch to ISS and loiter on station before integration into ABRS and germination | Shuttle middeck locker then ISS EXPRESS Rack (22 – 25 C)/3–25 days | OES Shuttle middeck locker/3–25 days on at least 24 hour delay from flight |
Active growth period each plate | ISS ABRS unit 22–24 C/12–14 days | OES ABRS unit 22–24 C/12–14 days on at least 24 hour delay from flight |
Photography and harvest of each plate to KFT | ISS work areas (22–24 C)/1–2 hrs | OES work areas (22–24 C)/1–2 hrs on at least 24 hour delay from flight |
RNAlater soak in KFT | ISS work areas (22–24 C)/12–24 hrs | OES work areas (22–24 C)/12–24 hrs on at least 24 hour delay from flight |
KFT Stowage on ISS | MELFI (between −35 and −95 C)/8–32 days | cryo freezer at KSC (−80 C)/8–32 days on at least 24 hour delay from flight |
Return to KSC laboratory | Double Cold Bag (−32 C)/2–3 days | cryo freezer at KSC (−80)/2–3 days |
Storage at KSC | cryo freezer at KSC (−80)/2–7 days | cryo freezer at KSC (−80)/2–7 days |
Transition to PIs’ laboratory | Transport on dry ice then to cryo freezer (−80) | Transport on dry ice then to cryo freezer (−80) |