Analysis of wild-type and 35S:: GbRLK transgenic Arabidopsis lines subjected to salt and drought treatment. (a) The response of transgenic Arabidopsis to salt and drought stress. Wild-type (Col-0) and transgenic Arabidopsis were grown in pots containing soil mixture (rich soil: vermiculite = 2:1, v/v) and maintained in a growth chamber at 22°C on a 16-h light/8-hr dark regimen. For drought stress treatment, the photograph was taken after withholding water for 42 days. For salt stress treatment, the photograph was taken after treatment with a 150 mM NaCl solution for 35 days at 22°C. The experiments were repeated three times with similar results. (b) and (c) represent the survival ratios of transgenic Arabidopsis lines and Col-0 (CK) under drought-stress treatment for 42 days and salt-stress treatment for 35 days, respectively. (d) Water loss rate in transgenic and non-transgenic plants; standard deviations (bars) are indicated. Comparisons were made between wild-type and individual transgenic lines under control or drought-stress conditions using the paired t-test. ** indicates significant differences compared with the control at P < 0.01.