Figure 3
RT-PCR assays confirm expression of differentially-regulated genes. (A) RT-PCR products generated using cDNA templates from RNA extracted from one of the microarray replicate experiments. ProOx; proline oxidase (Unigene Les.1610), F-box PP2-B15 (Unigene Les.23296), XET; xyloglycan endo-transglycosylase (Unigene Les.429), ACS1A; 1-aminocyclopropane-1-carboxylate synthase 1A (Unigene Les.1841). eIF4a; eukaryotic translation initiation factor 4a (Unigene Les.5856), is included as an internal loading control. (B) Plots of mean Affymetrix log2 expression signals from the three replicate microarray experiments for the corresponding genes shown in (A). Notation designates Pre-treatment/Treatment, where W = water, A = Alethea, S = salt.