Figure 8

Induction of PR1 and PDF1.2 following exogenous application of benzothiozidole and methyl jasmonate. A, Expression PR1 in response to benzothiozidole (BTH) treatment. B, expression of PDF1.2 in response to methyl jasmonate treatment. Plants were spray treated with either or 0.06% of Actigard® (Active ingradient: 50% w/w benzothiozidole) or 50 μM methyl jasmonate. Leaf tissues were harvested after 24 of spraying for total RNA extraction. Transcript levels of PDF1.2 and PR1 were normalized to the expression of GAPDH in the same samples and expressed relative to the normalized transcript levels of mock treated wild-type plants. The bars represent the mean and standard deviation from two independent experiments. Asterisks indicate significant difference in transcript levels compared to wild-type (Students t-test; P < 0.05).