Figure 5
Characterization of ETI in Atelp3-10 Plants. (A) Expression of nine ETI-inducible genes in Pst DC3000/avrRpt2-infected WT and Atelp3-10 plants. Plants were inoculated with Pst DC3000/avrRpt2 (OD600 = 0.001). Leaf tissues were collected at the indicated time points. Total RNA was extracted and analyzed for the expression of indicated genes using real-time qPCR. The y-axes indicate relative expression levels monitored by qPCR. Expression was normalized against constitutively expressed UBQ5. The x-axes indicate hours after Pst DC3000/avrRpt2 infection. Data represent the mean of three independent samples with SD. An asterisk (*) indicates that the expression level of the gene in the WT was significantly different from that in Atelp3-10 (p < 0.05, Student’s t-test). (B) Growth of Pst DC3000/avrRpt2 in WT, npr1, Atelp3-10, Atelp3-10 npr1, and rps2 plants. Plants were inoculated with Pst DC3000/avrRpt2 (OD600 = 0.0001). The in planta bacterial titers were determined immediately and three days postinoculation. Cfu, colony-forming units. Data represent the mean of eight independent samples with SD. Different letters above the bars indicate significant differences (p < 0.05, Student’s t-test).