Figure 1

Characterization of the gns2 Mutant. (A) Seeds of wild type (WT), npr1, and gns2 npr1 were placed on half-strength MS agar medium containing 0.26 mM SA. After three days of stratification, the plate was transferred to a growth chamber and photographed ten days later. (B) Four-week-old soil-grown WT, npr1, and gns2 npr1 plants were inoculated with the virulent bacterial pathogen Psm ES4326 (OD₆₀₀ = 0.001). Twenty-four hours later, the inoculated leaves were collected for SA measurement. Data represent the mean of three independent samples with standard deviation (SD). Different letters above the bars indicate significant differences (p < 0.05, Student’s t-test). (C) Total SA levels in Psm ES4326-infected WT, npr1, and gns2 npr1 plants. The experiment was carried out as in (B). SAG, 2-O-β-D-glucosylsalicylic acid. (D) Leaves of four-week-old soil-grown WT, npr1, and gns2 npr1 plants were inoculated with Psm ES4326 (OD₆₀₀ = 0.0001). The in planta bacterial titers were determined immediately and three days postinoculation. Cfu, colony-forming units. Data represent the mean of eight independent samples with SD. Different letters above the bars indicate significant differences (p < 0.05, Student’s t-test). (E) Morphology of npr1 and gns2 npr1 plants. The plants were grown on soil for four weeks before the photos were taken. The gns2 npr1 plant is a lighter shade of green than the npr1 plant.