Figure 4

Tissue-specific transcript abundance and phylogenetic analysis of N. attenuata nectarin1-like genes. (A) The transcript levels of three nectarin1-like genes in leaf, root, stem, corolla, and nectary of N. attenuata. The mean intensities (±SE) of NaNEC1a, b, c genes are plotted on the y-axis in a logarithmic scale. Nectary was divided into two stages on the base of its maturation and color: early white nectary (stage1) and late pink nectary (stage2). (B) Phylogenetic relationship among 10 germine-like proteins in N. attenuata (Na) and NECTARIN1 from the ornamental tobacco (Nicotiana langsdorffii x Nicotiana sanderae). Putative full-length amino acid sequences were aligned and phylogenetic tree was constructed by the UPGMA (unweighted pair group method with arithmetic mean) method. The numbers given for each branch reflect the numbers of amino acid substitutions per site. NEC, nectarin.