Figure 5

Loss of SPT function alters auxin accumulation but does not disrupt root patterning. (A, B) Confocal micrographs of 5 DAG roots stained with propidium iodide expressing PIN4p::PIN4-GFP. (A) Col-0. (B) spt-11. (C, D) Micrographs of 5 DAG roots expressing DR5::GUS. (C) Col-0. (D) spt-11. (E-J) Confocal micrographs of 5 DAG roots stained with propidium iodide. (E, F) pSCRp::GFP expression. Arrows indicate endodermal cells, asterisks indicate QC cells and arrowheads indicate vascular initials. (E) Col-0. (F) spt-11. (G, H) pSHR::GFP expression. The bracketed region is the stele. (G) Col-0. (H) spt-11. (I-J) pSHR::SHR-GFP expression. Arrows indicate endodermal cells, asterisks indicate QC cells and arrowheads indicate vascular initials. (I) Col-0. (J) spt-11. (K, L) Fluorescent brightener stained free hand cross sections of 7 DAG primary roots. Asterisks indicate the epidermal cell layer. Arrows indicate the cortical cell layer. Arrowheads indicate the endodermal cell layer. Cortical cells are numbered around the diameter. (K) Col-0. (L) spt-11. Scale bars in A-B and D-J indicate 100 μm. Scale bars in B-C and K-L indicate 50 μm.