Expression of MdANT1 and MdANT2 in thinned and un-thinned ‘Golden Delicious Smoothee’ (GS) fruit. Gene expression was analyzed from bloom to maturity. Dotted line represents the day of thinning (11 DAFB). Fold-change in the expression of a gene relative to its expression in thinned fruit at full bloom (0 DAFB) is presented here. MdACTIN and MdGAPDH were used as the reference genes in this study. Error bar represents the standard error of mean of four biological replicates (n = 4). Closed circles represent un-thinned fruit and open circles represent thinned fruit.