Figure 3
From: Analysis of the P1promoter in response to UV-B radiation in allelic variants of high-altitude maize
Kernel images, p1 alleles and estimation of the copy number of P1 genes in the different high altitude landraces. Individual kernel images from the different high altitude landraces analyzed, B73 and P1-rr; A: Arrocillo Amarillo; B: Cacahuacintle; C: Confite Puneño; D: Conico Norteño; E: Mishca (white pericarp); F: P1-rr and G: B73. The determination of the P1 copy number was performed by quantitative PCR analysis. The P-rr allele was used as a reference, and the ACT-1 gene was used as control of DNA template. Genomic DNA was quantified and each reaction was normalized using the Ct values corresponding to the Act-1 transcript. Location of primers used in shown in Additional file 1: Figure S1B. All measurements were done in triplicate using biological replicates.