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Figure 6 | BMC Plant Biology

Figure 6

From: Arabidopsis nonhost resistance gene PSS1confers immunity against an oomycete and a fungal pathogen but not a bacterial pathogen that cause diseases in soybean

Figure 6

The pss1 mutant was infected by fungal pathogen, F. virguliforme , but not by the bacterial pathogen, P. syringae pv. glycinea. A, Response of pss1 to F. virguliforme infection. Cell death and spread of mycelia stained with trypan blue were observed in infected seedlings of pss1 but not in those of Col-0 or pen1-1 following inoculation with F. virguliforme conidial spores. Single cell penetration by F. virguliforme was observed in pen1-1 but not in Col-0 seedlings. Red arrows show the germinating conidia. White arrow shows a dead infected cell. All images were taken 2 days post- inoculation and at 400X magnification. B, Responses of six P. sojae susceptible (pss1pss1) (S-4 through S-434) and six resistant (PSS1PSS1) (R-194 through R-332) F2:3 families and the pss1 mutant to inoculation with F. virguliforme conidial spores are presented. Data are the mean of three independent experiments. Error bars indicate S.E. among experiments. C, Response of pss1 to P. syringae pv. glycinea. Disease response in colony forming units (cfu) of six P. sojae susceptible (pss1pss1) (S-4 through S-434) and five resistant (PSS1PSS1) (R-194 through R-332) F2:3 families and the pss1 mutant 2 days following inoculation of intact leaves with P. syringae pv. glycinea are shown. Data are mean of three replications of a representative experiment. The experiment was repeated two times with similar results. Error bars indicate S.E. among experiments.

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