Immunolocalization of AtRanBPM in Arabidopsis cultured cells. A- Immunolocalization of endogenous AtRanBPM with affinity purified anti-AtRanBPM antibody. Signal for AtRanBPM was cytoplasmic with slight accumulation in the vicinity of nuclei and in dividing cells with slight enrichment in spindle and phragmoplast area. (AtRanBPM – green, chromatin – blue). B- Double immunofluorescence analysis of cells expressing GFP-AtRanBPM confirmed similar localization pattern for expressed GFP protein and for endogenous AtRanBPM protein (Figure 6A) (GFP-AtRanBPM – green, endogenous AtRanBPM – red, chromatin – blue). C- Double immunofluorescence analysis of AtRanBPM (green) and γ-tubulin (red) did not confirm colocalization of both proteins in interphase and in dividing cells. D- Double immunofluorescence of AtRanBPM (green) and Ran (red). Only a small portion of AtRanBPM is localized in nuclei compared to intensive nuclear signal for Ran protein. Bars = 10 μm.