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Table 2 Carbohydrate contents of LL-grown plants (A) as well as contents of adenylates in Col-0, adg1-1, tpt-2 and adg1-1/tpt-2 grown in LL (B) or HL (C)

From: Defects in leaf carbohydrate metabolism compromise acclimation to high light and lead to a high chlorophyll fluorescence phenotype in Arabidopsis thaliana

Plant lines Starch Sucrose Glucose Fructose
(μmol C6·g-1 fw)
A Plants grown in LL
Col-0 4.69 ± 0.59 b, d 1.71 ± 0.18 1.69 ± 0.48 b, d 0.53 ± 0.13
adg1-1 0.24 ± 0.06 a 1.47 ± 0.33 2.46 ± 0.14 a, c 1.05 ± 0.07
tpt-2 3.89 ± 0.47 b, d 1.10 ± 0.14 d 1.04 ± 0.07 b, d 0.32 ± 0.13
adg1-1/tpt-2 0.69 ± 0.54 a, c 1.36 ± 0.38 1.86 ± 0.16 a, c 0.68 ± 0.19
Plant lines ATP ADP AMP EC
(nmol·g-1 fw)
B Plants grown in LL
Col-0 34.0 ± 1.5 14.8 ± 1.1 1.3 ± 0.1 0.83
adg1-1 48.4 ± 13.5 21.7 ± 4.9 1.9 ± 0.6 0.82
tpt-2 30.1 ± 4.5 12.4 ± 2.0 1.1 ± 0.2 0.83
adg1-1/tpt-2 36.3 ± 0.4 16.9 ± 0.9 1.2 ± 0.1 0.82
Plant lines ATP ADP AMP EC
(nmol·g-1 fw)
C Plants grown in HL
Col-0 159.5 ± 10.5 90.8 ± 5.4 19.9 ± 1.8 d 0.76
adg1-1 191.9 ± 25.4 90.8 ± 8.6 14.9 ± 2.1 0.80
tpt-2 208.4 ± 7.9 106.3 ± 6.3 17.4 ± 3.1 d 0.79
adg1-1/tpt-2 154.5 ± 11.1 88.0 ± 4.7 9.0 ± 1.2 a, c 0.79
  1. Leaf samples were taken after 8 h in the light. Metabolism in the leaf samples was quenched immediately in N2 during illumination. The data represent the mean ± SE of n = 3 (A) or n = 6 (B and C) measurements. Significant differences in the data pairs were identified by the ANOVA test and further analyzed by the Tukey-Kramer test. The letters as superscripts denote the lines to which a significant difference (P < 0.05) was found with a = Col-0, b = adg1-1, c = tpt-2 and d = adg1-1/tpt-2. The energy charge (EC) was calculated from the relationship EC = ([ATP] + 0.5·[ADP])/([ATP] + [ADP] + [AMP]).