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Table 1 Chl-a fluorescence parameters as well as pigment and protein contents of the adg1-1/tpt-2 double mutant compared to the wild type (Col-0) and the adg1-1 and tpt-1 single mutants

From: Defects in leaf carbohydrate metabolism compromise acclimation to high light and lead to a high chlorophyll fluorescence phenotype in Arabidopsis thaliana

Lines Fv/Fmratio ΦPSII Chl content (mg·m-2) Chl a/bratio Carotenoid content (mg·m-2) Protein content (g·m-2)
A Plants grown in LL
Col-0 0.744 ± 0.011 0.584 ± 0.016 b, d 127.2 ± 7.5 2.68 ± 0.03 d 4.4 ± 0.3 0.59 ± 0.10
adg1-1 0.751 ± 0.010 0.487 ± 0.077 a, c 133.8 ± 16.6 2.64 ± 0.06 d 4.5 ± 0.6 0.57 ± 0.14
tpt-2 0.753 ± 0.019 0.582 ± 0.030 b, d 135.8 ± 11.3 2.61 ± 0.08 d 4.5 ± 0.3 0.44 ± 0.04
adg1-1/tpt-2 0.746 ± 0.005 0.472 ± 0.022 a, c 135.3 ± 9.2 2.35 ± 0.07 a, b, c 4.7 ± 0.4 0.54 ± 0.05
B Plants grown in HL
Col-0 0.800 ± 0.007 c, d 0.396 ± 0.014 d 286.2 ± 26.5 b, c, d 3.09 ± 0.13 d 10.6 ± 0.5 b, d 3.38 ± 0.42 b, d
adg1-1 0.778 ± 0.007 d 0.366 ± 0.014 d 320.6 ± 24.3a, c, d 3.00 ± 0.04 c, d 12.4 ± 0.9 a, c, d 4.29 ± 0.64 a, c, d
tpt-2 0.757 ± 0.030 a, d 0.359 ± 0.046 d 258.7 ± 9.9a, b, d 3.11 ± 0.07 b, d 10.1 ± 0.6 b, d 3.24 ± 0.42 b, d
adg1-1/tpt-2 0.226 ± 0.021 a, b, c 0.056 ± 0.027 a, b, c 103.0 ± 10.8a, b, c 2.13 ± 0.04 a, b, c 5.5 ± 0.5 a, b, c 2.26 ± 0.48 a, b, c
  1. The plants were grown for 4 weeks either in LL (A) or HL (B). Photosynthesis parameters were determined with the Imaging-PAM fluorometer using the integrated light source. Before the determination of Fv/Fm ratios the plants were dark-adapted for 15 min. The ΦPSII values refer to steady state photosynthesis, which was attained 7-10 min after onset of illumination with PDFs similar to those the plants experienced during growth (i.e. at 30 μmolm-2·s-1 [LL] and 300 μmol·m -2·s -1 [HL], respectively). The data represent the mean ± SE of n = 3 (Fv/Fm ratio, ΦPSII) or n = 5 (Chl content, Chl a/b ratio, carotenoid- and protein content) measurements. Significant differences in the data pairs were identified by the ANOVA test and further analyzed by the Tukey-Kramer test. The letters as superscripts denote the lines to which a significant difference (P < 0.05) was found with a = Col-0, b = adg1-1, c = tpt-2 and d = adg1-1/tpt-2.