Figure 4

Immunoblots of thylakoid proteins, spectroscopic determinations of functional PS components, and separation of PS complexes by Blue-Native gel electrophoresis. (A) Immunoblots of thylakoid proteins associated with photosynthesis after separation of total proteins (approximately 10 μg per lane) isolated from leaves of HL- and LL- grown Col-0 wild type, the adg1-1 and tpt-2 single mutants as well as the adg1-1/tpt-2 double mutant on SDS-PAGE. Note that the Coomassie staining for proteins of HL-grown tpt-2 corresponds to about 0.5-fold rather than 1-fold of Col-0 protein. P*-Threonin indicates signals obtained following incubation of the blots with a phospho-threonin antibody. The numbers indicate signals from PsbC (1), CaS (2), PsbA/PsbD (3), and LhcbII (4). (B) Spectroscopic determinations of functional components of PSII, PSI and the cyt b₆/f complex. (C) Immunoblots of PsbE in comparison to Lhcb2, Lhca1, and PsbE in HL-grown wild-type and adg1-1/tpt-2 plants. (D) Separation of thylakoid proteins of HL-grown Col-0 wild type, the adg1-1 and tpt-2 single mutants as well as the adg1-1/tpt-2 double mutant on Blue-Native gels. The numbers indicate individual protein fractions of the isolated thylakoids such as the NDH complex (1, see Additional file 2: Table S2), PSII super complexes (2), PSI supercomplex/PSII dimer (3), ATPase (4), PSII monomers (5), PSII/PsbC (6), and LHCII trimers (7)