Quantitative expression profiles of selected putative cytokinin regulatory genes during carpel and seed development in bread wheat A, TaIPT genes; B, TaCKX genes; C, TaZOG genes; D, TaGLU genes; E, TaRR genes. Data values are means of relative mRNA levels in fold changes detected using qRT-PCR, using at least two technical replicates for each of the two biological replicates. Error bars represent the SD calculated for the combined technical and biological replicates. GAPDH, β-actin, 18 S rRNA, and protein phosphatase gene (PP2A), were used as internal controls. Before quantification of the expression level of each of the target genes, the Ct numbers for each target gene were corrected by using the average correction factor determined for each of the four reference genes.