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Figure 5 | BMC Plant Biology

Figure 5

From: Inhibition of phenylpropanoid biosynthesis increases cell wall digestibility, protoplast isolation, and facilitates sustained cell division in American elm (Ulmus americana)

Figure 5

Protoplasts and developing cells obtained from American elm callus. Images depict American elm tissue cultured in MSO basal medium supplemented with 5 μM BA, 1 μM NAA, and 100 μM AIP after a 4 h digestion in cell wall degrading enzymes. a; Initial purified protoplast preparation stained with FDA for viability, cells fluorescing green indicate viability, b; Individual cell after 2 days of culture under visible light, c; Same cell as depicted in ‘b’ stained for cellulose with calcofluor white, blue fluorescence indicates cell wall formation, d; Protoplast that has completed one cell division 6 days after culture initiation, e; Cells depicted in ‘d’ stained with FDA for viability, f; Cells depicted in ‘d’ stained for cellulose with calcofluor white, g; cells depicted in ‘d’ stained for viability with FDA and cellulose with calcofluor white. h; Protoplast derived callus 14 days after culture initiation, i; Callus depicted in ‘h’ stained with FDA for viability, j; Callus depicted in h stained for cellulose with calcofluor white, k; Callus depicted in ‘h’ stained for viability with FDA and cellulose with calcofluor white.

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