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Table 1 Mutations identified in durum wheat SBEIIa

From: Development of high amylose wheat through TILLING

Gene

DNA

Protein

PSSM

SIFT

SBEIIaA

G5239A

G427D

6.6

0.09

 

C5256T

H433Y

22.3

0.00

 

G5267A

W436*

  
 

G5268A

D437N

7.9

0.04

 

G5429A

E461K

17.1

0.01

 

G5493A

G482E

27.1

0.00

 

C5801T

H518Y

-8.3

1.00

SBEIIaB

G5011A

G427D

-0.04

0.50

 

G5020A

R430H

21.4

0.00

 

G5022A

G431S

25.2

0.00

 

C5025T

H432Y

-3.6

1.00

 

G5033A

W434*

  
 

G5040A

D437N

19.9

0.01

 

G5062A

G444E

17.0

0.00

 

G5065A

S445N

-4.7

1.00

 

G5069A

W446*

  
 

G5073A

SJ

  
 

G5168A

R450K

19.0

0.01

 

G5189A

R457K

19.0

0.01

 

G5203A

E462K

18.3

0.00

 

G5219A

G467E

27.7

0.00

 

G5233A

G472R

27.3

0.00

 

G5234A

G472E

27.7

0.00

 

C5240T

T474I

21.9

0.00

 

G5272A

SJ

  
 

C5582T

A521V

4.8

0.33

  1. DNA and protein refer to the nucleotide and amino acid changes resulting from the mutation. SJ refers to a splice junction mutation and the symbol * indicates a stop mutation. Mutation severity is predicted using the PARSESNP and SIFT programs [36, 37]. Mutations are predicted to have a severe effect on protein function if PSSM scores are >10 and and SIFT scores are <0.05.